USS Jamestown may refer to any one of a number of United States Navy vessels.

, was a sloop that served from 1844 until 1892 , was a patrol gunboat that served from 1941 until 1946 , was originally a Liberty ship (AG-166) completed in 1945, converted and renamed in 1963, and then redesignated in 1964 to AGTR-3. Scrapped in 1970.