Holston may refer to:
Holston, Virginia Holston River Holston Formation Treaty of Holston Holston Conference, an annual conference within Methodism Holston Army Ammunition Plant in Kingsport, Tennessee The Holston, an NRHP-listed high-rise in Knoxville, Tennessee
People with the surname Alan Holston, British fashion entrepreneur David Holston (born 1986), American basketball player Mike Holston (born 1958), American football player