Middelburg may refer to:
Places and jurisdictions Europe Middelburg, Zeeland, the capital city of the province of Zeeland, southwestern Netherlands Roman Catholic Diocese of Middelburg, a former Catholic diocese with its see in the Zeeland city, Netherlands Middelburg, South Holland, a small village near Reeuwijk, Netherlands Middelburg, Belgium
South Africa Middelburg, Mpumalanga, a large industrial and farming town on the Highveld in South Africa Middelburg, Eastern Cape, a town in the Great Karoo, South Africa
Asia-Pacific Middelburg Bastion, in the Fortress of Malacca, Malaysia Middelburg Island, former name of 'Eua, an island of Tonga
People with the surname Jack Middelburg (1952–1984), Dutch motorcycle road racer Paul of Middelburg (1446–1534), Belgian Roman Catholic scientist and bishop
Others Middelburg (ship), 18th century Dutch ship