Indican is a colourless organic compound, soluble in water, naturally occurring in Indigofera plants. It is a precursor of indigo dye.
Chemical reactions Indican is a glycoside. Its most significant reaction is hydrolysis of to yields β-D-glucose and indoxyl. Beta-glucosidase, a common enzyme, catalyzes this process. Because the hydrolysis is slow in the absence of the enzyme, indican can be viewed as a protected version of indoxyl. Once formed, the indoxyl is oxidized by atmospheric oxygen to give blue indigo dye. Since synthetic indigo is produced on a massive scale by chemical routes, i.e. 50,000 tons/y (2011), the prospect of a biological pathway is of practical interest.
Medical significance
Biosynthesis A reaction, similar to that used to produce indigo dye, is seen in the normal population,Urinary Excretion of Indoxyl Sulfate (Indican) by Human Subjects Ingesting a Semisynthetic Diet Containing Variable Quantities of l-Tryptophan - BRYAN 19 (2): 113 - American J... who excrete small amounts of the chemical in their urine. Normal urine reacting to hydrogen peroxide does at times produce a bluish tinge. Tryptophan is first converted to indole (which is excreted in faeces), then to indican by bacteria in the gut. Indican, being water-soluble, is then excreted through the urine. Following absorption from the gut, indole is converted to 3-hydroxy indole (indoxyl or indican) in the liver, where it is again then conjugated with sulfuric acid or glucuronic acid through normal xenobiotic metabolism pathways. It is then transported to the kidneys for excretion.Urine Indican Test Bio Center Lab tests Urine Metabolism – Indican
The enzyme "indoxyl esterase" has been found in humans and is involved in another pathway of chemical reactions involving indoxyl.
Pathology Individuals affected by blue diaper syndrome exhibit a defect in tryptophan metabolism. Tryptophan is first converted to indole, then to indican by bacteria in the gut. Indican is then excreted into the urine and from there into the diaper where, upon exposure to air, it is oxidised by atmospheric oxygen to indigo blue dye.
Indican interferes with many commercial procedures for measuring total bilirubinIndican interference with six commercial procedures for measuring total bilirubin - Poon and Hinberg 31 (1): 92 - Clinical Chemistry which can be a problem for renal failure patients whose blood indican levels are raised. It can cause gastrointestinal symptoms in patients whose protein absorption is reduced, as in Hartnup's disease, allowing for greater bacterial decomposition of the tryptophan to indole and its conversion to indican.
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