Line 54 (), also known as the Gein Line (), is a line of the Amsterdam Metro system. It runs between Amsterdam Centraal in the city center and Gein metro station in the southeastern neighborhood of Gaasperdam. The line forms the southern branch of the Oostlijn (East Line).

The initial segment of the line, between Weesperplein and Holendrecht, opened on 14 October 1977, replacing the former bus line 55. An extension to Amsterdam Centraal was completed on 11 October 1980. The final segment, from Holendrecht to Gein, opened on 27 August 1982.

Line 54 is long and includes 15 stations. It runs underground through central Amsterdam, emerging to the surface as it follows NS railway lines. After Holendrecht, the line branches off onto its own dedicated right-of-way leading to Gein.

Until 2013, the line was primarily operated using M1, M2, and M3 rolling stock, which were built between 1973 and 1980 specifically for the Oostlijn. These units were gradually phased out and replaced by the M5 series between 2013 and 2015. The M7 series later entered service beginning in 2023.

Service + Train frequency Time period Frequency Rush hour and daytime 10 minutes Early morning (before 07:00), evenings (after 20:00), and weekends (before 10:00) 15 minutes Evenings (until 20:00) and weekends (after 10:00) 12 minutes Midsummer rush hour and daytime 12 minutes Midsummer early morning, evenings, and weekends 15 minutes

Station list Station Transfers Borough Centraal Station Centrum Nieuwmarkt Waterlooplein Weesperplein Wibautstraat Oost Amstel Spaklerweg Ouder-Amstel Van der Madeweg Duivendrecht Zuid Strandvliet Zuidoost Bijlmer ArenA Bullewijk Holendrecht Reigersbos Gein

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