DFO may refer to:
Businesses and organizations Dairy Farmers of Ontario, Canada Department of Fisheries and Oceans, Canada Direct Factory Outlet, Australia German Women's Order (Deutscher Frauenorden or DFO), merged into the National Socialist Women's League
Science 1,8-Diazafluoren-9-one, a chemical that reveals fingerprints Deferoxamine, medication for iron overdose Dispersive flies optimisation, a swarm intelligence algorithm
Other uses Dungeon Fighter Online, a 2009 computer game