Some botanical gardens in the Czech Republic have collections consisting entirely of Czech Republic native and endemic species; most have a collection that include plants from around the world. Czech Republic botanical gardens and arboreta are administered by local governments, some are privately owned.

List of botanical gardens Brno Botanical gardens and arboretum of Mendel University of Agriculture and Forestry in Brno Botanical gardens of the Faculty of Natural Sciences of Masaryk University Hradec Králové, Botanical gardens of medicinal plants of the Pharmaceutical Faculty of Charles University Kostelec nad Černými lesy, Arboretum of the Czech Agricultural University in Prague Liberec, Liberec botanical gardens Olomouc Palacký University of Olomouc Botanical gardens Olomouc Exhibition Centre of Flora Ostrava, Botanical gardens of the Faculty of Natural Sciences of Ostrava University Plzeň Plzeň zoological and botanical gardens Sofronka Arboretum of the Forestry and Game Management Research Institute (VÚLHM) Prague Praha 2, Na Slupi, Botanical gardens of the Faculty of Natural Sciences of Charles University Praha 8, Troja, Botanical gardens of the City of Prague Praha 9, Malešice botanical gardens Průhonice Botanical department of the Czech Academy of Sciences Dendrological gardens of the Silva Tarouca Research Institute for Landscape and Ornamental Horticulture Rakovník, Botanical gardens of the Rakovník College of Agriculture Tábor, Botanical gardens of the Tabor Higher Specialist School and College of Agriculture Teplice, Botanical gardens of the City of Teplice

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