Transmembrane protein 254 is a transmembrane protein that is encoded by the TMEM254 gene, it is predicted to have many orthologs across eukaryotes.
Gene
TMEM254 is located on the long arm of chromosome 10, open reading frame 57 on the positive strand. It is found at position 10q22.3. It has seven known transcript variants.
This image shows the cytogenetic band of human chromosome 10 and the various gene locations on it. TMEM254 can be seen at the thin red band with the box around, placed at q22.3.
mRNA
TMEM254 consists of ten total exons and is 13,912 bp in length.
Isoforms TMEM254 has 3 isoforms produced by alternative splicing. Isoform Length Mass (Da)112314,2432849,692310312,068
Protein
TMEM254 contains: 147 amino acids. Five transmembrane domains Molecular weight 16.4 kilodaltons Isoelectric point 9.39 domain of unknown function 4499 (DUF4499) region The secondary structure of TMEM254 is predicted to consist of alternating pairs of alpha helices and beta sheets.
Expression
According to a human GEO profile and human and mouse EST profiles, TMEM254 appears to be expressed at a relatively high level in most tissues.EST Profile Viewer- HumanEST Profile Viewer- Mouse
TMEM254 is highly expressed in reproductive structures, and a wide range of cells. These include, but are not limited to, thyroid gland, pancreas, kidney, skin, breasts, and testes.
Homology There are no known paralogs for transmembrane protein 254, however it does have various orthologs within orthologs within eukaryotes. The following table presents some of the orthologs found using searches in BLAST. A comparison of a wide variety of known TMEM254 orthologs meant to display the diversity of species for which orthologs are found is listed in the table below: Organism Common name Divergence from Humans (MYA) Accession number Sequence length Sequence identity Sequence similarity NotesHomo sapiensHumansN/ANP_001257296N/A100%100%MammaliaPan troglodytesChimpanzee6.40XP_0033126956.499.3%99.3%MammaliaRhinopithecus bietiBlack snub-nosed monkey28.1XP_01188626028.195.2%97.3%MammaliaChrysemys pictabelliiPainted Turtle320XP_00816875332046.3%57.8%ReptiliaEsox luciusNorthern Pike320NP_001290628 432 45%60.4%Actinopterygii Alligator mississippiensisAmerican Alligator320KYO27663 320 43.3%56.7%ReptiliaNanorana parkeriHigh Himalaya frog353XP_018428933 353 42.3%57.7%AmphibiaPhaethon lepturusWhite-tailed Tropicbird320XP_01028644632038.8%59.3%AvesHippocampus comesTiger Tail Seahorse432XP_01973741343238.1%56.5%ActinopterygiiCallorhinchus miliiElephant Shark465XP_00789741546537.9%58.2%ChondrichthyesPatagioenas fasciata monilisBand-tailed Pigeon320OPJ8464632036.7%59.9%AvesSaccaglossus kowalevskiiAcorn Worm627XP_00682128562731.8%51%Enteropneusta
Predicted Post-Translational Modification
Using various tools at ExPASyExPASy Proteomics Server the following are possible post-translational modifications for TMEM254. 4 possible PKC phosphorylation sites 3 possible PKA phosphorylation sites
References