Tolomato can refer to:
Nuestra Señora de Guadalupe de Tolomato, also called Mission Tolomato, a Spanish Christian mission in Georgia, in Spanish Florida, in the colonial era. Tolomato Cemetery, a cemetery established in the Nuestra Señora de Guadalupe de Tolomato. Tolomato River, part of the Intracoastal Waterway in Florida. Guana Tolomato Matanzas National Estuarine Research Reserve