The following railroads operate in the U.S. state of West Virginia.
Common freight carriers Appalachian and Ohio Railroad (AO) Beech Mountain Railroad (BEEM) CSX Transportation (CSXT) Durbin and Greenbrier Valley Railroad (DGVR) operates Durbin Railroad and West Virginia Central Railroad (WVC) Elk River Railroad (ELKR) Kanawha River Railroad (KNWA) Norfolk Southern Railway (NS) R.J. Corman Railroad/West Virginia Line (RJCV) South Branch Valley Railroad (SBVR) Wheeling and Lake Erie Railway (WE) Winchester and Western Railroad (WW)
Private freight carriers Belpre Industrial Parkersburg Railroad (BIP) Kanawha River Terminal Railroad (KRT) Little Kanawha River Rail, Inc. (LKRR) Short Line Services operates Big Eagle Rail, LLC, lessor of Kanawha Rail Corporation
Passenger carriers
Amtrak (AMTK) Cass Scenic Railroad Durbin and Greenbrier Valley Railroad MARC Potomac Eagle Scenic Railroad South Branch Valley Railroad
Defunct railroads