The following railroads have operated in the State of Alabama.
Class I Railroads BNSF Railway (BNSF) Canadian National Railway (CN) through its subsidiary Illinois Central Railroad (IC) CSX Transportation (CSXT) Norfolk Southern Railway (NS) including its subsidiaries Alabama Great Southern Railroad (AGS) and Central of Georgia Railroad (CG)
Regional Railroads Alabama and Gulf Coast Railway (AGR)
Shortline and Terminal Railroads Alabama and Tennessee River Railway (ATN) Alabama Southern Railroad (ABS) Alabama Warrior Railway (ABWR) Autauga Northern Railroad (AUT) Bay Line Railroad (BAYL) Birmingham Terminal Railway (BHRR) CG Railway (CGR) Columbus and Chattahoochee Railroad (CCH) Conecuh Valley Railroad (COEH) Eastern Alabama Railway (EARY) Georgia Southwestern Railroad (GSWR) Huntsville and Madison County Railroad Authority (HMCR) Luxapalila Valley Railroad (LXVR) Meridian and Bigbee Railroad (MNBR) Mississippi Central Railroad (MSCI) Sequatchie Valley Railroad (SQVR) Tennessee Southern Railroad (TSRR) Terminal Railway Alabama State Docks (TASD) Three Notch Railroad (TNHR) Wiregrass Central Railroad (WGCR)
Passenger Railroads Amtrak (AMTK): The Crescent and Mardi Gras Service
Heritage and Scenic Railroads North Alabama Railroad Museum (Mercury and Chase Railroad) Heart of Dixie Railroad Museum (Calera and Shelby Railroad)
Industrial Rail Operations Rapid Switching Services (switching at Kerr-McGee at Mobile) Southern Electric Railroad Company (SERC; Southern Company plants; trackage rights granted to Norfolk Southern)
Defunct railroads