The Marquis of Villanueva del Fresno () is a hereditary title in the Spanish nobility. This marquisate was bestowed by Carlos I to , for services rendered to the Crown.
The Seigniory of Villanueva del Fresno (Spanish: Señorío de Villanueva del Fresno) traces its origins to the year 1332, when Alfonso XI conceded to Martín Fernández Portocarrero the village of Villanueva del Fresno, and granted him the title 1st Lord of Villanueva del Fresno and 2nd Lord of Moguer.
List of marquesses Marquises of Villanueva del FresnoCreated by Charles I of Spain Titleholder PeriodIJuan Portocarrero1530-1544IIPedro Portocarrero1544-1557IIIAlonso Portocarrero1557-1560IVJuan Portocarrero1560-?VAlonso Portocarrero¿-1622VIFrancisca Luisa Portocarrero1622-1639VIIJuan Gaspar Domingo Portocarrero de Moscoso1639-1640VIIIFrancisco Portocarrero1640-?IXAlonso PortocarrerounknownXPedro Fernández Portocarrero¿-1703XIDiego Antonio-López Barradas1718-1727XIIAntonio-López Barradas Portocarrero1727-1729XIIICristóbal Gregorio Portocarrero1729-1763 XIVCristóbal Pedro Portocarrero?-1757XVMaría Francisca de Sales Portocarrero y Zúñiga 1763-1808 1765-1798XVIEugenio Portocarrero y Palafox Diego Fernández de Velasco1808-1834 1798-1811XVIICipriano de Palafox y Portocarrero1834-1839 XVIIIMaría Francisca de Sales Portocarrero y Kirkpatrick1839-1860XIXCarlos María Fitz-James Stuart y Portocarrero1881-1901XXJacobo Fitz-James Stuart y Falcó1901-1955XXIMaría del Rosario Cayetana Fitz-James Stuart y Silva1955-2014XXIICarlos Fitz-James Stuart y Martínez de Irujo2014-present
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