Coiled-Coil Domain Containing protein 82 (CCDC82) is a protein that in humans, is encoded for by the gene of the same name, CCDC82. The CCDC82 gene is expressed in nearly all of human tissues at somewhat low rates. As of today, there are no patents involving CCDC82 and the function remains unknown.
Gene
CCDC82 is located on chromosome 11 at 11q21.5. It contains two domains of unknown function, DUF4196 and DUF4211. The DNA sequence is 37,155 base pairs long and contains 7 exons.
Homology
CCDC82 is present in many orthologs. It is conserved throughout other mammals, reptiles, birds and bony fish. It is not found in invertebrates, bacteria or fungi. There are no paralogs.
Scientific name Common name Date of divergence Accession number Length Percent identity Percent similarity Pan troglodytes Chimpanzee 6.3 Mya XP_001147806.1 544 aa 60.8 99 Gorilla gorilla gorilla Gorilla 8.8 Mya XP_004052053.1 521 aa 56.2 91 Pongo pygmaeus Orangutan 15.7 Mya NP_003253075.1 343 aa 95.3 98 Nomascus leucogenys Crested gibbon 20.4 Mya XP_003253075.1 554 aa 57.8 94 Papio anubis Olive baboon 29 Mya XP_003910631.1 542 aa 52 86 Callithrix jacchus Marmoset 42.6 Mya XP_002754732.1 526 aa 51.2 86 Mus musculus Mouse 92.3 Mya NP_079810.2 518 aa 39.7 74 Rattus norvegicus Brown rat 92.3 Mya NP_001007661.1 516 aa 37.4 71 Canis lupus familiaris Dog 94.2 Mya XP_542232.2 520 aa 46 79 Bos taurus Cow 94.2 Mya NP_001039559.2 522 aa 42.4 74 Ailuropoda melanoleuca Giant panda 94.2 Mya XP_002925755.1 528 aa 45.4 80 Loxodonta africana African bush elephant 98.7 Mya XP_003415705.1 521 aa 40.4 75 Sarcophilus harrisii Tasmanian devil 162.2 Mya XP_003764344.1 518 aa 36.9 70 Monodelphis domestica Gray short-tailed opossum 162.2 Mya XP_001363143.1 516 aa 36.7 72 Ornithorhynchus anatinus Platypus 167.4 Mya XP_001511067.1 505 aa 26.6 70 Gallus gallus Chicken 296 Mya XP_423807.3 460 aa 24.3 56 Meleagris gallopavo Wild turkey 296 Mya XP_003203546.1 462 aa 21.5 70 Taeniopygia guttata Zebra finch 296 Mya XP_002198267.1 575 aa 20 61 Anolis carolinensis Carolina anole 296 Mya XP_003219357.1 603 aa 19.9 49 Xenopus tropicalis Western clawed frog 371.2 Mya XP_002935613.1 462 aa 21 73
mRNA
Promoter
The predicted promoter for CCDC82 is located on the minus strand and spans from base pairs 96,122,963 to 96,123,587. It is 625 base pairs long.
Transcription factors
The transcription factors listed below are for the predicted promoter sequence and are located on the minus strand. Detailed Family Information Span Score Alternative splicing variant of FOXP1 48-64 1.00 Homeodomain transcription factor Otx2 34-50 .992 Hypoxia-response Elements 111-127 .985 Homeobox A10/HOX 1.8 52-68 .957 SRY box 9 80-104 .947 Mesoderm posterior 1 and 2 42-62 .937 c-Myc/Max heterodimer 44-60 .929 SAM pointed domain containing ets transcription factor 27-47 .923 cAMP-responsive element binding protein 134-154 .917 PR domain zinc finger protein 14 138-152 .912
Protein
The protein it encodes for is 344 amino acids in length. The protein itself is very acidic and is very rich in aspartic acid and glutamic acid. It is also very deficient in alanine, containing only two alanines in the entire sequence. The alanines are located adjacent to each other, amino acid number 233 and 234. Alanine 233 is highly conserved throughout the orthologs. The molecular weight is 40.0 kdal and the isoelectric point is 4.383
Expression
CCDC82 is found in nearly all tissues in the human body, however it is present in higher quantities in the skeletal muscles, adrenal cortex, and the trigeminal ganglion.
Post translational modifications
CCDC82 has several predicted phosphorylation sites. There are 32 predicted serine phosphorylation sites, 5 threonine, and 3 tyrosine.
Interactions
CCDC82 is known to interact with two proteins. It indirectly interacts with VHL, a gene that encodes for a tumor suppressor and ubiquitin protein ligase. It also interacts with EWSR1, which functions as a transcriptional repressor.
Clinical significance
CCDC82 is a circulat-responsive gene. Circulat is a product designed to restore systemic vascular health. It is a plant based product and taken by patients who suffer from diabetes or circulatory problems.
Possible function
Based on the information that CCDC82 is affected by the Circulat product it could be hypothesized that CCDC82 is involved in circulatory function. However, this is purely speculation.
References
External links