Acireductone synthase (EC number 3.1.3.77, E1, E-1 enolase-phosphatase) is an enzyme with systematic name 5-(methylsulfanyl)-2,3-dioxopentyl-phosphate phosphohydrolase (isomerizing). It catalyses the following overall reaction:
The enzyme characterised from Klebsiella pneumoniae and rat liver hydrolyses 5-(methylsulfanyl)-2,3-dioxopentyl phosphate to 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one and orthophosphate (Pi). This is part of the methionine salvage pathway. The crystal structure of the enzyme showed that the mechanism involves an enol of the starting diketone.
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