3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase (, HsaA) is an enzyme with systematic name . This enzyme catalyses the following chemical reaction:

3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O

This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone.

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