Erythromycin 12 hydroxylase (, EryK) is an enzyme with systematic name erythromycin-D,NADPH:oxygen oxidoreductase (12-hydroxylating) . This enzyme catalyses the following chemical reaction
The enzyme characterised from Saccharopolyspora erythraea converts erythromycin D into erythromycin C by introduction of a hydroxy group at the C-12 position of the macrocycle. It uses reduced nicotinamide adenine dinucleotide phosphate (NADPH) and oxygen as cofactors. Erythromycin C is subsequently converted to the antibiotic, erythromycin A, by the enzyme erythromycin 3''-O-methyltransferase.
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