James, Jim, or Jimmy Johnstone may refer to:
Law and politics Sir James Johnstone, 3rd Baronet (1697–1772), Scottish MP Sir James Johnstone, 4th Baronet (1726–1794), Scottish MP James Johnstone (1801–1888), Scottish MP
Nobility James Johnstone, 1st Earl of Hartfell (1602–1653), Scottish peer James Johnstone, 2nd Marquess of Annandale (c. 1687–1730), Scottish peer James Hope-Johnstone, 3rd Earl of Hopetoun (1741–1816), Scottish peer
Sports Jim Johnstone (umpire) (1872–1927), American baseball umpire Jimmy Johnstone (1944–2006), Scottish footballer Jim Johnstone (basketball) (born 1960), American basketball player James Johnstone (rugby union) (born 1990), Scottish rugby union player
Others Chevalier de Johnstone (James Johnstone, 1719–c. 1791), Scottish Jacobite army officer James Johnstone (antiquary) (died 1798), Scandinavian antiquarian James Johnstone (physician) (1730?–1802), Scottish physician James Johnstone (explorer) (1759–1823), British naval officer and explorer James Johnstone (publisher) (1815–1878), British newspaper proprietor James Johnstone (stock breeder) (1859–1933), New Zealand businessman and stock breeder James Johnstone (biologist) (1870–1932), British biologist and oceanographer Jim Johnstone (poet) (born 1978), Canadian physiologist and poet
See also James Johnston (disambiguation) James Johnson (disambiguation)