John Harley may refer to:
John Harley (bishop, died 1558) (died 1558), earlier bishop of Hereford John Harley (bishop, died 1788) (1728–1788), dean of Windsor and bishop of Hereford John Pritt Harley (1786–1858), English comic actor John Harley (physician) (1833–1921), English physician, geologist, and botanist John Harley (footballer) (1886–1960), Scottish-born Uruguayan footballer John Brian Harley (1932–1991), geographer, cartographer, and map historian John B. Harley (died 1846), president of Fordham University, 1843–1845 Jack Harley (John Laker Harley, 1911–1990), British biologist