Route 408 or Highway 408 may refer to:
Canada Manitoba Provincial Road 408
Costa Rica National Route 408
Japan Japan National Route 408
United States Florida State Road 408 Georgia State Route 408 (unsigned designation for Interstate 475 Louisiana Highway 408 Maryland Route 408 Maryland Route 408 New York: New York State Route 408 New York State Route 408A (former) New York State Route 408 (former) County Route 408 (Albany County, New York) County Route 408 (Erie County, New York) Pennsylvania Route 408 Puerto Rico Highway 408 Texas State Highway Spur 408 Virginia State Route 408 (former)