Guanosine phosphorylase () is an enzyme that catalyzes a reversible phosphorolysis reaction
In the forward direction shown, the two substrates of the enzyme characterised from Escherichia coli are guanosine and orthophosphate (Pi). Its products are α-D-ribose 1-phosphate and guanine. It has also been found in rabbit.
This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is guanosine:phosphate alpha-D-ribosyltransferase.
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