The enzyme trans-L-3-hydroxyproline dehydratase () catalyzes the chemical reaction

trans-L-3-hydroxyproline Δ1-pyrroline 2-carboxylate + H2O

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is ''trans-L-3-hydroxyproline hydro-lyase (Δ1-pyrroline-2-carboxylate-forming). This enzyme is also called trans''-L-3-hydroxyproline hydro-lyase.

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