VLB may refer to:
Science and technology VESA Local Bus, a local bus based on the Intel 80486 CPU Very large board, a large printed circuit board Vincaleukoblastine, or vinblastine, an antitumor alkaloid
Other uses VLB Berlin (Versuch- u. Lehranstalt für Brauerei in Berlin), a brewing school and trade organization in Berlin Volunteer Life Brigade, the forerunners of today Auxiliary Coastguard in the United Kingdom